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KMID : 1149220020100020143
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Korean Journal of Oriental Medical Prescription
2002 Volume.10 No. 2 p.143 ~ p.158
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Effects of Jakyakgamchotang Extract on the Trachea Smooth Muscle
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Kook Yoon-Bum
Lee Jang-Cheon
Kim Hee-Soo
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Abstract
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The purpose of the present study is to determine the effect of Jakyakgamchotang on histamine or acetylcholine induced tracheal smooth muscle contraction in rats and guinea pigs. Guinea pig(500g, male) and Sprague Dawley rats(250g, male) were killed by CO2 exposure and a segment (4-5mm) of the thoracic trachea from each rat and guinea pig was cut into equal segments and mounted ’in pairs’ in a tissue bath. Contractile force was measured with force displacement transducers under 0.5g loading tension. The dose of histamine(His) which evoked 50% of maximal response(ED50) was obtained from cumulative dose response curves for histamine (10-7 ~ 10-4M). Contractions evoked by His (ED50) were inhibited significantly by Jakyakgamchotang. In guinea pig tracheal smooth muscle, the mean percent inhibition of histamine induced contraction was 90.8% (p〈0.001) after 100㎕/ml were inhibited significantly by Jakyakgamchotang. In guinea pig tracheal smooth muscle, the mean percent inhibition of histamine induced contraction was 90.8% (p〈0.001) after 100㎕/ml Jakyakgamchotang. Propranolol indomethacin and methylene blue(10-7M) slightly but significantly attenuated the inhibitory effects of Jakyakgamchotang. These results indicate that Jakyakgamchotang can relax histamine or acetylcholine induced contraction of guinea pig and rat tracheal smooth muscle.
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KEYWORD
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Jakyakgamchotang, smooth muscle contraction thoracic trachea contractile force, histamine, acetylcholine
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